Serum 8,12-iso-iPF2α-VI Isoprostane Marker of Oxidative Damage and Cognition Deficits in Children with Konzo

摘要: We sought to determine whether motor and cognitive deficits associated with cassava (food) cyanogenic poisoning were high concentrations of F2-isoprostanes, well-established indicators oxidative damage. Concentrations serum F2-isoprostanes quantified by LC-MS/MS anchored measures proficiency performance, which respectively assessed through BOT-2 (Bruininks/Oseretsky Test, 2 nd Edition) KABC-II (Kaufman Assessment Battery for Children, edition) testing 40 Congolese children (21 konzo 19 presumably healthy controls, overall mean age (SD): 9.3 (3.2) years). Exposure cyanide was ascertained its main metabolite thiocyanate (SCN) in plasma urine. Overall, SCN ranged from 91 325 172 1032 mmol/l urine, respectively. Serum isoprostanes 0.1 0.8 (Isoprostane-III), 8.3 (total Isoprostane-III), 1.5 (Isoprostane-VI), 2.0 9.0 Isoprostane-VI), or 0.2 1.3 ng/ml (8,12-iso-iPF2a-VI isoprostane). Children poorly performed at the relative (p,0.01). Within regression models adjusting age, gender, proficiency, other biochemical variables, 8,12-iso-iPF2a-VI isoprostane significantly performance (b= 232.36 (95% CI: 251.59 213.03; P, 0.001). This model explained over 85% variation score konzo, but not significant explaining impairment. These findings suggest that and, possibly, brain injury is mediated part damage konzo. appears be a good marker neuropathogenic mechanisms may used monitor impact interventional trials prevent neurotoxic effects poisoning.