Biomarkers of oxidative stress study V: ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine.

摘要: Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma urine rats was studied as a continuation international Biomarker Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal to identify biomarker for ozone-induced oxidative stress assess whether inconsistent results often reported literature might be due limitations available methods measuring various types products. time- dose-dependent effects ozone rat lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, tyrosine- phenylalanine products, well urinary malondialdehyde F2-isoprostanes were investigated with techniques. criterion used recognize marker model that significant could identified measured biological fluid seen at both doses more than one time point. No statistically differences between experimental control groups either dose point this study. Tissue samples not included. Despite all work accomplished BOSS study ozone, no product has yet met required criteria being biomarker. current negative findings consequence are great importance, because they document complex systems, present vivo experiment, assays may provide meaningful data especially human studies.