An hypothesis on the role of cellular colloid osmotic pressure in determining behavior of cells in vitro including anchorage dependency and maintenance of the differentiated state

摘要: The osmotic problems involved when cells are isolated from tissues analyzed. Evidence is considered which indicates that in vivo the Na pump operating at maximal or near rates and this depends on low leak for salts water due to various aspects of structure. Dispersion tissue results breakdown these barriers free diffusion cell subjected an enormous increase passive influx colloid pressure without being able its pumping rate extent needed maintain volume control. It proposed primary problem faces vitro compensate effective pressure, e.g. excess, emerging with finding most normal have adhere a surface order grow “anchorage dependency” analyzed terms way adhesion spreading result changes ion movements into enabling them achieve fluid balance face excess. also differentiated state more dependent than proliferation. failure conditions used culture adequately excess limiting amount protein can be synthesized, dissipation cellular energy, orientation components contribute directly loss differentiation occurs during growth vitro.