Synchronous nanoscale topographic and chemical mapping by differential-confocal controlled Raman microscopy

摘要: Confocal Raman microscopy is currently used for label-free optical sensing and imaging within the biological, engineering, physical sciences as well in industry. However, these methods have limitations, including their low spatial resolution poor focus stability, that restrict breadth of new applications. This paper now introduces differential-confocal controlled a technique fuses differential confocal spectroscopy, enabling point-to-point collection three-dimensional nanoscale topographic information with simultaneous reconstruction corresponding chemical information. The microscope collects scattered light together Rayleigh light, both reflected (these are normally filtered out conventional systems). Inherent design instrument significant improvement axial focusing topographical features image (to ∼1  nm), which, when coupled super-resolution restoration, gives lateral 220 nm. By using controlling imaging, system presents enhancement measurement accuracy, precision, stability (with an antidrift capability), mitigating against thermal vibrational artefacts. We also demonstrate improved scan speed, arising consequence nonaxial scanning mode.