Dipole‐like backscatter stimulated Raman scattering for in vivo imaging

摘要: Stimulated Raman scattering (SRS) scanning microscopy has the potential to enable label-free in vivo imaging for research and clinical medicine. Volume SRS from focus occurs forward scattered direction. Therefore, multiple events are required direct light out of tissue, reducing depth resolution. Here, a method called Stokes interference (SISRS) is introduced that operates by addition standard pump stimulated emission probe beams third beam donut beam. The close wavelength and, after passage through π phase plate, forms an annular focal plane with bright nodes above below focus. beats beam, when they destructively interfere each other, microscope's 3-D spot reduced subwavelength dimensions. A volume emits dipole pattern backscatter lobes, enabling high-resolution single-backscatter deep within tissues. reduction also increases resolution image creating beyond diffraction limit. SISRS may provide images comparable achieved stained vitro tissues all planes section. Copyright © 2014 John Wiley & Sons, Ltd.