[Isolation and culturation, phenotype detection of rat bone marrow mesenchymal stem cells].

摘要: Aim To investigate the more effective methods of isolation, culture mesenchymal stem cell (MSC), and examine surface phenotype MSC. MSC were separated from rats bone marrow by plastic adherence purified controlling time digestion combined with adhesion separation, proliferated in flasks that coated I collogen. Methods The morphology studied phase contrast microscope; cycles forth generation tested flow cytometry identified using immunocytochemical methods. Results Primary cultured oval, spindle-shaped or polygonal, adhered to within 24 h reached 90% confluence 7-8 days. After purification proliferation, they uniformly long form passaged every five rate hours was all. showed 80% cells at G0/G1 phase. Immunocytochemistry positive for CD29, CD105, CD166, VLA-4 P-selectin, while negative CD34 CD45. Conclusion obtained our experiments purified, expanded rapidly, expressed some molecules. protocol should make it possible undertake a large number future application.