Molecular and Immunological Characterization of the Fimbriae of Porphyromonas gingivalis

摘要: Shigeyuki Hamada*, Taku Fujiwara, Seiji Morishima, Ichiro Takahashi, Nakagawa, Shigenobu Kimura, and Tomohiko Ogawa Department of Oral Microbiology, Osaka University Faculty Dentistry, Suita, 565, Japan Received September 12, 1994 Key words: Porphyromonas gingivalis, Fimbriae, fimA gene, Synthetic peptide, Periodontitis1. Introduction A wide variety bacteria including Gram-posi-tive Gram-negative ones possess proteinaceous, hair-like appendages called fimbriae or pili. Fim-briae, which are macromolecular structures express-ed on the surface layer various pathogenic bacteria, frequently found to mediate attach-ment organism mucosal epithelial cells animal hosts, those gastrointesti-nal, respiratory genitourinary tracts oral cavity (41). For example, some strains Escherichia coli preferentially adhere urinary (65), while Bordetella pertus-sis, Haemophilus influenzae Streptococcus pyogenes attach upper tract (5, 7, 36). Thus, this process has been postulated be essential in pathogenesis infectious diseases at sites, although it is only one many processes leading colonization disease. In addition, indigenous organisms play an important role establishment maintenance normal mi-crobial floras. bacterial recog-nized ubiquitous characteristics for ini-tial cell-to-surface cell-to-cell adherence host that may eventually lead microbial invasion into tissues (6, 21, 57). The provides solid surfaces microniches become colo-nized by microorganisms. composition flora mouth differs from site different times ages. sub-gingival other sites mouth. Evidence indicates number cultivable species subgin-gival exceeds 300, among predomi-nant types anaerobic, a motile organ-isms, especially when inflammation occurs gin-gival periodontal areas (20, 37). Periodontal nature induce inflammatory response gingival underlying supportive tissue accumulation crevice. This complex both mi-crobiologically immunologically (10). Current evidence implicates black-pigmented anaerobic rods, as being most associated with active adult periodontitis (34, 66), ctinobacillus actinomycetemcomitans, microaer-ophilic rod, mainly juvenile (60). significant, but limited species, certain Prevotella, Treponema, Fusobacterium, Selenomonas, Bacte-roides, Eikenella, etc. associate per-iodontal disease (62). Earlier electron microscopic observations P. gingivalis related rods revealed these exhibited fine fibrillar (fimbriae) were arrang-ed peritrichously, emerging cell (19, 29, 32, 33). short review will focus