Purification and characterization of a novel secondary fimbrial protein from Porphyromonas gingivalis strain 381

摘要: We previously reported the existence of two different kinds fimbriae expressed by Porphyromonas gingivalis ATCC 33277. In this study, we isolated and characterized a secondary fimbrial protein from strain FPG41, fimA-inactivated mutant P. 381. FPG41 was constructed homologous recombination technique using mobilizable suicide vector, failed to express long (41-kDa fimbriae) that were produced on cell surface However, short structures observed electron microscopy. The purified DEAE–Sepharose CL-6B column chromatography. eluted at 0.15 M NaCl, molecular mass approximately 53 kDa as estimated SDS-PAGE. An antibody against 53-kDa reacted with wild-type strain. 41-kDa 67-kDa 33277 did not react same antibody. Moreover, N-terminal amino acid sequence showed only 2 15 residues identical those protein. These results show properties are well fimbriae.