Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species.
作者： Tomohiko Ogawa , Tetsu Mukai , Kenji Yasuda , Hidetoshi Shimauchi , Yoshihisa Toda
DOI: 10.1111/J.1399-302X.1991.TB00504.X
关键词: Microbiology 、 Polyclonal antibodies 、 Fimbria 、 Molecular biology 、 Monoclonal antibody 、 Antibody 、 Gel electrophoresis 、 Porphyromonas gingivalis 、 Epitope 、 Antigen 、 Biology
摘要: Rabbit polyclonal antibody (Poly-1) and mouse monoclonal antibodies (mAbs) TO-11, TO-14 TO-M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly-1 the 3 mAbs screened their reactivity with whole cells oral nonoral black-pigmented bacterial species by enzyme-linked immunosorbent assay (ELISA) binding experiment using [125I]Poly-1 [125I]mAbs. ELISA revealed that definitely reacted of all 11 strains P. tested. However, 8 TO-M1. These results confirmed radiolabelled mAb TO-11 to strains. The M(r) fimbrial subunit protein (fimbrilin) isolated purified from 381, BH18/10, HW24D-1, 6/26 OMZ 314 was 41 kDa sodium dodecylsulfate-polyacrylamide gel electrophoresis. It found immunoblotting TO-14/TO-M1 recognized different epitopes Immunoelectron micrographs visualized similar serotype-specific bindings fimbriae. indicate could be divided into at least 2 separate groups based on immunochemical specificities
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sci-hub.se 参考文章(29)
R. B. Taylor, N. R. Klinman, General methods for the study of cells and serum during the immune response: the response to dinitrophenyl in mice. Clinical and Experimental Immunology. ,vol. 4, pp. 473- 487 ,(1969)
F Nakazawa, J J Zambon, H S Reynolds, R J Genco, Serological studies of oral Bacteroides intermedius. Infection and Immunity. ,vol. 56, pp. 1647- 1651 ,(1988) , 10.1128/IAI.56.6.1647-1651.1988
S Hanazawa, K Saitoh, Y Ohmori, H Nishihara, S Fujiwara, S Kitano, Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Bacteroides gingivalis. Infection and Immunity. ,vol. 46, pp. 285- 287 ,(1984) , 10.1128/IAI.46.1.285-287.1984
David M. Neville, Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System Journal of Biological Chemistry. ,vol. 246, pp. 6328- 6334 ,(1971) , 10.1016/S0021-9258(18)61792-2
R Parent, C Mouton, L Lamonde, D Bouchard, Human and animal serotypes of Bacteroides gingivalis defined by crossed immunoelectrophoresis. Infection and Immunity. ,vol. 51, pp. 909- 918 ,(1986) , 10.1128/IAI.51.3.909-918.1986
S Fujimura, T Nakamura, Isolation and characterization of a protease from Bacteroides gingivalis. Infection and Immunity. ,vol. 55, pp. 716- 720 ,(1987) , 10.1128/IAI.55.3.716-720.1987
T Ogawa, H Shimauchi, S Hamada, Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally. Infection and Immunity. ,vol. 57, pp. 3466- 3471 ,(1989) , 10.1128/IAI.57.11.3466-3471.1989
R.J. Genco, J.J. Zambon, L.A. Christersson, The Origin of Periodontal Infections Advances in Dental Research. ,vol. 2, pp. 245- 259 ,(1988) , 10.1177/08959374880020020901
A J van Winkelhoff, N Kippuw, J de Graaff, Serological characterization of black-pigmented Bacteroides endodontalis. Infection and Immunity. ,vol. 51, pp. 972- 974 ,(1986) , 10.1128/IAI.51.3.972-974.1986
Jergen Slots, Max A. Listgarten, Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases Journal of Clinical Periodontology. ,vol. 15, pp. 85- 93 ,(1988) , 10.1111/J.1600-051X.1988.TB00999.X