Genetic selection and liquid medium conditions improve the yield of androgenetic plants from diploid potatoes
作者： H. Uhrig
DOI: 10.1007/BF00251187
关键词:
摘要: Solatium tuberosum L. diploid strains with superior androgenetic capacity have been selected for from progenies of unselected material. The paper also demonstrates that the use a liquid medium culturing potato anthers, instead conventional solid agar plates, improves yield embryoids. new method, associated two successive cycles selection response, allows induction and regeneration microspore derived plants on large scale. best genotype (clone 21 in this paper) regenerates frequency around 30 per each anther plated. Over 80% regenerated are diploid. It is suggested embryoids mainly originate unreduced microspores by mechanism which maintains heterozygous or partly genetic situation.
springer.com
sci-hub.se 参考文章(15)
Niels O. Frandsen, The plastid number as a character in potatoes Theoretical and Applied Genetics. ,vol. 38, pp. 153- 167 ,(1968) , 10.1007/BF00933811
Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Verlag.. ,(1977) , 10.1007/978-3-662-02279-5
Elfriede M. Linsmaier, Folke Skoog, Organic Growth Factor Requirements of Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 18, pp. 100- 127 ,(1965) , 10.1111/J.1399-3054.1965.TB06874.X
HERBERT M. RUBINSTEIN, Coagulant action of proteolytic enzymes. Nature. ,vol. 180, pp. 1202- 1203 ,(1957) , 10.1038/1801202A0
G. Wenzel, O. Schieder, T. Przewozny, S. K. Sopory, G. Melchers, Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theoretical and Applied Genetics. ,vol. 55, pp. 49- 55 ,(1979) , 10.1007/BF00285189
R. W. HOUGAS, S. J. PELOQUIN, A Haploid Plant of the Potato Variety Katahdin Nature. ,vol. 180, pp. 1209- 1210 ,(1957) , 10.1038/1801209A0
Sudhir K. Sopory, Evert Jacobsen, Gerhard Wenzel, Production of monohaploid embryoids and plantlets in cultured anthers of Solanum tuberosum Plant Science Letters. ,vol. 12, pp. 47- 54 ,(1978) , 10.1016/0304-4211(78)90038-X
G. Wenzel, H. Uhrig, Breeding for nematode and virus resistance in potato via anther culture. Theoretical and Applied Genetics. ,vol. 59, pp. 333- 340 ,(1981) , 10.1007/BF00276445
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
B. Foroughi-Wehr, H. M. Wilson, G. Mix, H. Gaul, Monohaploid plants from anthers of a dihaploid genotype of Solanum tuberosum L. Euphytica. ,vol. 26, pp. 361- 367 ,(1977) , 10.1007/BF00026996