Gene expression signatures in peripheral blood cells from Japanese women exposed to environmental cadmium.
作者： Satoru Dakeshita , Tomoko Kawai , Hirokazu Uemura , Mineyoshi Hiyoshi , Etsuko Oguma
DOI: 10.1016/J.TOX.2008.12.004
关键词:
摘要: Abstract The objective of this study was to examine the effects environmental cadmium (Cd) exposure on gene expression profile peripheral blood cells, using an original oligoDNA microarray. population consisted 20 female residents in a Cd-polluted area (Cd-exposed group) and non-Cd-polluted individually matched for age (control group). mRNA levels Cd-exposed subjects were compared with those respective controls, microarray containing probes 1867 genes. Median Cd concentrations (3.55 μg/l) urine (8.25 μg/g creatinine) from group 2.4- 1.9-times higher than control group, respectively. Microarray analysis revealed that significantly up-regulated 137 genes down-regulated 80 genes, group. Ingenuity Pathway Analysis Application (IPA) differentially expressed likely modify oxidative stress mitochondria-dependent apoptosis pathways. Among five positively correlated or urine. Quantitative real-time PCR (RT-PCR) validated significant up-regulation CASP9 , TNFRSF1B GPX3 HYOU1 SLC3A2 SLC19A1 SLC35A4 ITGAL down-regulation BCL2A1 COX7B . After adjustment differences background characteristics two groups, we finally identified seven Cd-responsive ( ), all which constituted network controls response by IPA. These may be marker useful health risk assessment chronic low level Cd.
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