Identification of the lipooligosaccharide biosynthetic gene cluster from Mycobacterium marinum

摘要: Lipooligosaccharides (LOSs) are antigenic glycolipids that present in some species of Mycobacterium including the Canetti strain M. tuberculosis. The core LOS structures from several mycobacterial organisms have been established, but biosynthetic pathways LOSs remain unknown. In this study, we describe two transposon insertion mutants marinum exhibit altered colony morphology. Cell wall analysis reveals MRS1271 mutant is defective synthesis LOS-II, whereas MRS1178 accumulates an intermediate between LOS-I and -II. genetic lesions were localized to genes, MM2309 MM2332. encodes a UDP-glucose dehydrogenase involved d-xylose. MM2332 predicted encode decarboxylase. These genes previously identified losA gene cluster likely be biosynthesis LOSs. Our results also show play important role sliding motility, biofilm formation, infection host macrophages. Taken together, our studies identified, for first time, locus. This step assessing differential distribution among understanding virulence.