Definition of the surface antigens of Mycobacterium malmoense and use in studying the etiology of a form of mycobacteriosis.

摘要: Mycobacterium malmoense is the latest of a roster atypical mycobacteria implicated in pulmonary infections. Yet it lacks recognizable phenotypic features to allow its ready identification. Some 23 clinical isolates M. were examined for homologous seroagglutination reactions and characteristic surface antigens. One group showed concordant agglutination interreactions an identical spectrum glycolipids are regarded as sensu stricto. The newly found, trehalose-containing lipooligosaccharide class. De-O-acylation followed by high-pressure liquid chromatography revealed one major several minor oligosaccharides. Partial acidic cleavage release glycosidically linked trehalose, alpha-mannosidase digestion demonstrate presence non-reducing-end mannobiose, perdeuteriomethylation, partial acid hydrolysis, reduction, O ethylation, combined with 1H nuclear magnetic resonance electron impact fast-atom bombardment mass spectrometry structure oligosaccharide alpha-D-Manp-(1----3) -alpha-D-Manp-(1----[2-alpha-L-Rhap-(1--]4--3)-alpha-L-Rh ap- (1----3)-alpha-D-Glcp-(1----1)-alpha-D-Glcp, which two 2-alpha-L-Rhap residues methylated at C-3. (Man, mannose; Rha, rhamnose; Glc, glucose; p, pyranosyl). structures oligosaccharides also determined; they differ distal nonreducing end. dominant was acylated octanoate, 2-methyleicosanoate, 2,4-dimethylpentacosanoate yield species-specific antigen malmoense, we regard most feature pathogen.