Prenatal Stress and Peripubertal Stimulation of the Endocannabinoid System Differentially Regulate Emotional Responses and Brain Metabolism in Mice

摘要: The central endocannabinoid system (ECS) and the hypothalamic-pituitary-adrenal-axis mediate individual responses to emotionally salient stimuli. Their altered developmental adjustment may relate emergence of emotional disturbances. Although environmental influences regulate phenotype throughout entire lifespan, their effects result particularly persistent during plastic stages (e.g. prenatal life adolescence). Here, we investigated whether stress – in form gestational exposure corticosterone supplemented maternal drinking water (100 mg/l) last week pregnancy combined with a pharmacological stimulation ECS adolescence (daily fatty acid amide hydrolase URB597 i.p. administration - 0.4 mg/kg between postnatal days 29–38), influenced adult mouse behaviour brain metabolism measured through vivo quantitative magnetic resonance spectroscopy. Compared control mice, URB597-treated subjects showed, short-term, reduced locomotion and, long term, motivation execute operant obtain palatable rewards paralleled by levels inositol taurine prefrontal cortex. Adult mice exposed showed increased behavioural anxiety elevated zero maze, (increased glutamate hippocampus; N-Acetyl-Aspartate hypothalamus). Present data further corroborate view that persistently adulthood. Yet, whilst hypothesized these factors be interactive nature, observed consequences were independent from those drug-induced adolescence.