Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry

作者: Suk Weon Kim , Sung Hee Ban , Ook Joon Yoo , Jang Ryol Liu

DOI: 10.1007/S11816-007-0006-7

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摘要: Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in vacuum, and has been widely applied to the discrimination closely related microbial strains. Minimally prepared samples embryogenic non-embryogenic calluses derived from various higher plants (sweet potato, morning glory, Korean ginseng, Siberian balloon flower) were subjected PyMS for spectral fingerprinting. A dendrogram unweighted pair group method, with arithmetic mean pyrolysis spectra, divided into ginseng callus others, which subsequently groups, regardless plant species derived. In group, was agreement known taxonomy plants. These results indicate that analysis could be discriminating capacity taxonomic classification.

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