Synthesis of an HIV-1 Tat transduction domain-rotavirus enterotoxin fusion protein in transgenic potato

摘要: A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP490) was transferred Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene detected in the genomic of transformed plant leaf tissues PCR amplification. Tat-NSP490 identified tuber extracts immunoblot analysis using anti-NSP490 and anti-Tat as primary antibodies. Enzyme-linked immunosorbent assay results showed that made up 0.0015% total soluble protein. synthesis potato demonstrates feasibility cell delivery domain carrier for non-specific targeting antigens mucosal immune system.