Identification of nucleolus organizer chromosomes and frequency of active NORs in river buffalo (Bubalus bubalis L.)

摘要: SUMMARYBlood cells from 22 river buffaloes (Bubalus bubalis L., 2n = 50) raised in the South Italy were cultured for both early—and late—incorporation of BrdU so as to obtain G- and R-banding, respectively. Slides sequentially treated G-banding/Ag-NOR R-banding/Ag-NOR techniques. These procedures allowed identify which chromosomes carry NORs frequency active per NO-chromosome. According standard nomenclature, buffalo are located at telomeres 3p, 4p, 6, 21, 23 24 while frequencies NO-chromosome higher (64.8%, 70.2% 72.2%, respectively) than 6 21 (42.1%, 17.3% 28.1%, respectively).