Comparative genomic hybridization for cytogenetic evaluation of stillbirth.

摘要: Abstract Objective: To ascertain the feasibility and reliability of comparative genomic hybridization for cytogenetic evaluation macerated stillbirths. Materials: We examined ten stillborn fetuses above 15 weeks’ gestation whose karyotypes were unknown because tissue culture failure. Sixteen that successfully karyotyped using prenatal or postnatal tissues also as controls, including five pregnancy terminations with autosomal aneuploidy, one sex chromosome a chromosomal deletion; normal karyotypes, three aneuploidy discrepancy between chorionic villi fetus. Results: All analyses in fresh successful except one. aneuploidies confirmed. Comparative failed fetus deletion short arm 18. In without known aberrant profile was found; however, results not confirmed interphase fluorescence situ hybridization. triploidy diagnosed DNA flow cytometry. Conclusion: is valuable backup technique screening from when fails. Gains losses can subsequently be by hybridization, probes focus on specific loci chromosome.