Overcoming problems with limiting DNA samples in forensics and clinical diagnostics using multiple displacement amplification

摘要: The availability of DNA samples that are adequate quality and quantity is essential for any genetic analysis. fields forensic biology clinical diagnostic pathology testing often suffer from limited yield insufficient material to allow extensive This study examined the utility a recently introduced whole genome amplification method termed Multiple Displacement Amplification (MDA) amplifying variety sample types commonly encountered in diagnostics. MDA reaction, which employs highly processive bacteriophage φ29 polymerase, was found generate high molecular weight template suitable downstream applications low copy number down single level. cells yielded sufficient up 20,000,000 PCR assays, allowing further confirmatory on quantities or archiving precious future work. of degraded using identified requirement successful synthesis product templates. Furthermore, products comparative genomic hybridisation (CGH) assays presence bias. However, this bias overcome by introducing novel modification protocol. Future directions work include investigations into short tandem repeat (STR) human identifications application modified protocol cell abnormalities.