Molecular cloning of two human cellular retinoic acid-binding proteins (CRABP). Retinoic acid-induced expression of CRABP-II but not CRABP-I in adult human skin in vivo and in skin fibroblasts in vitro.

摘要: Cellular retinoic acid binding proteins (CRABP) are low molecular weight whose precise function remains unknown. To investigate the role of CRABP in human skin we have cloned CRABP-II cDNA as well coding region CRABP-I. The predicted amino sequences CRABP-I and demonstrated a 99.3 93.5% identity to mouse CRABP-II, respectively. transcripts were undetectable adult epidermis by RNA blot hybridization, while probe detected an approximately 1.2-kilobase mRNA transcript. External application 0.1% cream vivo for 16 h resulted 16-fold induction transcripts, remained undetectable. Expression but not mRNA, was also markedly increased (greater than 15-fold) treatment fibroblasts cultured from skin, whereas no significant observed lung fibroblasts. Human significantly agents which known induce keratinocyte differentiation vitro. marked inducibility gene is compatible with idea that this isoform important acid-mediated regulation growth differentiation.