Identification and independent regulation of human mesangial cell metalloproteinases

摘要: Identification and independent regulation of human mesangial cell matrix metalloproteinases. Mesangial cells are known to secrete metalloproteinases that capable degrading the constituents GBM, hence potentially involved not only in regular maintenance ECM components glomerulus, but also contributing any damage these occurs disease states. In this report we positively identify by Northern blotting neutral proteinase is constitutively secreted (HMC) as gelatinase A (MMP2). Stimulation HMC IL-1β or PMA causes an increase total amount gelatinolytic activity secreted. On examination, however, increased shown, both immunoreactivity PCR be due induction higher molecular weight form gelatinase, B (MMP9), while remained unaffected. addition antigen messenger RNA have been identified for specific inhibitors TIMP-1 TIMP-2. The appearance larger inducible with similar substrate specificity implies turnover may released pathological situations becomes predominant. synthesis secretion TIMP-2 indicates controlling its own enzymes.