Immobilized redox enzymatic catalysts: Baeyer–Villiger monooxygenases supported on polyphosphazenes
作者： Aníbal Cuetos , Ana Rioz-Martínez , María L. Valenzuela , Iván Lavandera , Gonzalo de Gonzalo
DOI: 10.1016/J.MOLCATB.2011.10.002
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摘要: Abstract A novel method has been employed for the selective covalent co-immobilization of a Baeyer–Villiger monooxygenase (phenylacetone from Thermobifida fusca) and NADPH recycling enzyme (glucose-6-phosphate dehydrogenase) on same polyphosphazene carrier first time starting {NP[O2C12H8−x(NH2)x]}n (x ranging 0.5 to 2) using glutaraldehyde as connector. In all cases preparation was active it found that optimum proportion amino groups in per monomer. The immobilized biocatalysts showed similar selectivity when compared with isolated monooxygenase, demonstrating potential this type immobilizing material, although their recyclability must still be improved.
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