Gene delivery to rat and human Schwann cells and nerve segments : a comparison of AAV 1-9 and lentiviral vectors
作者： S A Hoyng , F De Winter , S Gnavi , L van Egmond , C L Attwell
DOI: 10.1038/GT.2015.47
关键词:
摘要: Schwann cells (SCs) in an injured peripheral nerve form pathways for regenerating axons. Although these initially support regeneration, SCs lose their pro-regenerative properties following a prolonged period of denervation. Gene transfer to SC can enhance therapeutic potential. In this article, we compared adeno-associated viral (AAV) vectors based on serotypes 1-9 capability transduce cultured primary rat and human segments. AAV1 is the best serotype SCs, whereas AAV2 AAV6 performed equally well SCs. Transduction monolayers did not accurately predict transduction efficiency Rat segments could be genetically modified by set four AAV (AAV1, AAV5, AAV7, AAV9), was superior The current experiments were undertaken as first step towards future clinical implementation ex vivo AAV-based gene therapy surgical repair. entirely different serotypes, documented here, highlights one challenges translating from experimental animals patients.
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