Genomic Diversity of Three Brazilian Native Food Crops Based on Double-Digest Restriction Site-Associated DNA Sequencing

作者: Alessandro Alves-Pereira , Mariana Novello , Gabriel Dequigiovanni , José Baldin Pinheiro , Pedro H. S. Brancalion

DOI: 10.1007/S12042-019-09229-Z

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摘要: The megabiodiversity of Brazil created opportunities for the domestication a number crop species, including some major global importance. Considering economic value many Brazilian native crops, genetic characterization their populations is fundamental to support utilization and conservation resources, currently threatened by deforestation intensification monuculture exotic crops. Recent advances in DNA sequencing technologies have promoted rapid genomic evaluation non-model those only local In this context, we evaluated diversity three crops: manioc (Manihot esculenta), annatto (Bixa orellana) jucara palm (Euterpe edulis). Double-digest restriction site-associated (ddRAD) was employed identify thousands SNP markers each species (1952 manioc, 3362 1040 jucara). Population analyses identified loci putatively under selection, but unavailability genome sequences hampers further these Nonetheless, were effective population structure. levels inbreeding compatible with biology species. While wild cultivated remarkably genetically divergent, same not observed between accessions annatto, differentiation among samples from different environments. application approaches may be valuable establishment better practices management promoting valorization resources.

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