Alloreactive T Cells That Do Not Require TCR and CD8 Coengagement Are Present in Naive Mice and Contribute to Graft Rejection

摘要: Class I alloreactive CTL populations have been defined as either CD8 dependent or independent, based upon their ability to kill target cells in the presence of Ab CD8. The CD8-dependent population uses a coreceptor role with TCR, and mutations class molecule that destroy binding site abrogate killing, even if cell expresses other allelic forms molecules an intact for CD8-independent apparently does not require CD8, has no effect on these appropriate cells. We isolated third is inhibited by addition yet can express alloantigenic incapable provided also non antigenic contain refer bystander-dependent CTL. Many (10 12) were able H-2Kb-expressing transfectants T2 cells, consistent idea they recognize peptide-independent determinant may be expressed at high density surface. These are only readily detectable vitro when spleen from mice primed vivo skin graft used.