Modulation of neurite outgrowth in neuroblastoma cells by protein kinase C and platelet-activating factor.
作者： K. Herrick-Davis , G. Camussi , F. Bussolino , C. Baglioni
DOI: 10.1016/S0021-9258(18)55108-5
关键词:
摘要: Previous reports have shown that thrombin and activators of protein kinase C (PKC) inhibit neurite outgrowth (NOG) in neuroblastoma cells cultured serum-free medium. Therefore, we tested the hypothesis PKC activation mediates effect on NOG murine NB-2a cells. After 2 h medium, 70% displayed neurites; addition 300 ng/ml reduced to 24% within 1 h. This inhibition was after were pretreated for 24 with 200 nM phorbol dibutyrate down-regulate PKC. Thrombin 12-myristate 13-acetate inhibited an additive way inhibitors H-7, H-8, HA1004 reversed a rank order activity consistent inhibition. Furthermore, translocated from cytosol membrane-bound form 5 10 min thrombin. These findings indicate inhibits through PKC-dependent pathway. stimulates synthesis phospholipid platelet-activating factor (PAF) some However, markedly stimulated when PAF or its analogue carbamyl-PAF added medium serum. receptor antagonist SRI 63072 accumulated kinetics similar inducPAF synthesized by de novo pathway, as incorporation [3H]choline. suggest is mediator neither nor these
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