Variation at position 86 of the pfmdr1 gene in samples from an area with seasonal transmission in eastern Sudan

摘要: Malaria is the most common parasitic disease of humans worldwide. A factor that aggravates many attempts to control epidemiologic malaria situation spreading resistance against anti-malarial drugs. In this project point mutation at position 86 Plasmodium. falciparum multidrug gene (pfmdr1), which thought contribute Chloroquine resistance, was analysed in 188 samples from a low transmission area eastern Sudan, where endemicity seasonal. The patient group studied had asymptomatic and sub patent parasitemia persisted during transmission-free dry season, after being treated with Chloroquine. To differentiate between wild type mutant genotypes, nested PCR restriction fragment length polymorphism enzyme Apo1 used. Out 79 (42%) were successfully analysed. Of those, 72% parasites genotypes or mixed infection. No conclusions on relevance pfmdr1 are made due remaining gaps. However, eventual sources error previous findings study discussed.