Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins

摘要: Ligand affinity chromatography separation is based on unique interaction between the target analyte and a ligand, which coupled covalently to resin. It simple, rapid, selective, efficient purification procedure of proteins providing tens thousands fold in one step. The biological activity isolated retained most cases thus function revealed concomitantly with isolation. Prior completion genome project this method facilitated rapid reliable cloning corresponding gene. Upon project, partial protein sequence enough for retrieving its complete mRNA hence sequence. This indispensable isolation both expected (e.g. receptors) but mainly unexpected, unpredicted very much surprising binding proteins. No other approach would yield latter. chapter provides examples proteins, from rich sources human as well unexpected found by serendipity.