The Effect of Cyclosporin A on Free Radical Processes and Interactions with Antioxidants Badanie wpływu cyklosporyny A na procesy wolnorodnikowe i jej interakcji z antyoksydantami

摘要: Background. Previous study of a group patients treated with CsA after renal transplantation showed statisti− cally elevated concentration lipid peroxidation products, measured as the MDA + 4−HNE level in blood plasma. Objectives. Evaluating whether is responsible for increase or hydroxyl radical ge− neration by an vitro method. Moreover, authors investigated two important lipophilic antioxidants, coenzyme Q10 and vitamin E, could be considered protective reparative agents free processes cau− sed CsA. Material Methods. Mitochondria isolated from human placenta were used experimental model. content was TBARS method, theOH deoxirybose degradation, protein concen− tration Lowry The influence doses 30-750 ng/ml on andOH investigated. Also evaluated (antioxidant given before CsA) effects CoQ10 E oxidative stress caused Coenzyme con− centrations, 1.5 3.0 µg/ml, three concentrations 1.5; 5.0, 15.0 µg/ml. Results. examinations that 450-750 increased mitochon− dria statistically significant way. A positive linear correlation, Pearson's coefficient r = 0.6030 (p 0.000), between dose obtained. ElevatedOH formation highest applied CsA, 750 ng/ml. at µg/ml protected against high significance, but it did not repair. elevatedOH decreased both used, unlike which (1.5, 15 µg/ml) acted agent, decreasing OH for− mation < 0.01). Vitamin inhibited ways, observed (15 most effective protection. Conclusion Cyclosporin can induce radicals processes, generation. investigations point out possibility reducing negative these antioxidants. usefulness mitochondrial model analysis estimated (Adv Clin Exp Med 2006, 15, 3, 435-443).