Transcriptional activation of breast cancer-associated gene 2 by estrogen receptor.
作者： Fathima R. Kona , Karri Stark , Luke Bisoski , Daniela Buac , Qiuzhi Cui
DOI: 10.1007/S10549-012-2107-4
关键词:
摘要: RNF115, or Breast Cancer-Associated Gene 2 (BCA2), encodes a RING-finger ubiquitin E3 ligase, expression of which was associated with estrogen receptor (ER)-positive status in human breast tumors. Although the BCA2 promoter contains several response element (ERE) half-sites, role ER regulation transcription has not been reported. The aim this study is to investigate molecular mechanism by regulates transcription. mRNA and protein levels were examined RT-PCR Western blot analysis, respectively, localization assessed immunofluorescence. activity E2 tested dual luciferase reporter assay binding chromatin immunoprecipitation assay. We found that are regulated ER-positive MCF7 cancer cells MDA MB 231 stably transfected ER. Estrogen treatment hormonal depleted 231/ER resulted increased nuclear cytoplasmic staining. Cycloheximide able inhibit levels, suggesting potential at transcriptional level. Anti-estrogens like tamoxifen ICI 182 178 counteracted E2-induced knockdown siRNA significant decrease lower pattern. lead increase response, ERE region promoter. therefore newly identified target receptor.
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