Development of tools for the genetic manipulation of Pseudomonas aeruginosa
作者： Sang-Jin Suh , Laura A Silo-Suh , Dennis E Ohman
DOI: 10.1016/J.MIMET.2004.03.018
关键词:
摘要: To facilitate study of the opportunistic bacterial pathogen Pseudomonas aeruginosa, several genetic tools were developed. These include a series cassettes carrying (a) minimal sequence for origin transfer (oriT) RP4 plasmid introducing into P. aeruginosa via conjugation, (b) replicon (stabilizing fragment or SF) maintenance plasmids in and (c) transcriptionally non-polar tetracycline resistance gene (TcR) insertional mutagenesis. Additional constructs (d) two conjugative suicide lacZ reporter fusion studying expression at transcriptional translational level, (e) gentamicin resistant promoter-probing mini-Tn5 lacZ, (f) tightly regulated T7 promoter/repressor system to control aeruginosa.
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