Quantitative In Situ Measurement of Estrogen Receptor mRNA Predicts Response to Tamoxifen
作者： Jennifer M. Bordeaux , Huan Cheng , Allison W. Welsh , Bruce G. Haffty , Donald R. Lannin
DOI: 10.1371/JOURNAL.PONE.0036559
关键词:
摘要: Purpose Quantification of mRNA has historically been done by reverse transcription polymerase chain reaction (RT-PCR). Recently, a robust method detection utilizing in situ hybridization described that is linear and shows high specificity with low background. Here we describe the use AQUA quantitative immunofluorescence (QIF) for measuring using ESR1 (the estrogen receptor alpha gene) breast cancer to determine its predictive value compared Estrogen Receptor α (ER) protein.
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