An airtight approach to the inebriometer: from construction to application with volatile anesthetics.

摘要: The mechanism by which inhaled anesthetics work is not fully understood, although they have been extensively used. Much research has done showing the likelihood that there more than one pathway or of action. A long-term goal our laboratory to decipher these mechanisms using Drosophila melanogaster, an excellent model organism for this purpose. In order do this, we modified and constructed apparatus called inebriometer quantitatively analyze response flies anesthetics. While new fly community, updated design provides a relatively low-labor, high-throughput means performing screens in search genes involved anesthetic mechanism. Here describe construction airtight designed We also provide data validates establishes procedure its use.