Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia.

摘要: Laboratories that study Drosophila melanogaster or other insects commonly use carbon dioxide (CO2) anaesthesia for sorting work. Unfortunately, the of CO2 has potential unwanted physiological effects, including altered respiratory and muscle physiology, which impact motor function behaviours. The effects at different levels exposure times were examined on subsequent recovery as assessed by climbing flight assays. With little a five minute to 100% CO2, D. exhibited deficits up 24 hours after exposure. Any length over minutes produced lasted days. Flight behaviour was also impaired following Overall, there positive correlation between time both Furthermore, 65% affected capability melanogaster. These negative are due CO2-specific mechanism an anoxic effect. results indicate heretofore unconsidered behavioural tests revealing importance monitoring accounting when performing studies in insects.