CHANGES IN ENZYME ACTIVITIES DURING THE PROGAME PHASE IN PETUNIA HYBRIDA

摘要: Summary Pollen tubes grow through the stigma and style after pollination of spermatophyta, whereupon fertilization can take place in ovules. During growth hundreds pollen style, an interaction occurs between tissue tubes. This finds expression various kinds metabolic changes which are coupled with alterations enzyme activities. Some these activities examined following compatible Petunia hybrida. The acid phosphatases exhibit a decrease activity pollination. The other investigated enzymes show increase. Following increase uridine diphosphate glucose dehydrogenase aspartate carbamoyltransferase be ascribed to characteristic There is small alanine aminotransferase, aminotransferase (AsAT) glutamate (deamination) (GDH deamin.) that cannot caused by alone. Large increases carbohydrate metabolism place, viz. ketose-l-phosphate aldolase (KPA) citrate synthase. An important for amino synthesis, (amination) amin.) shows also great pollination. It has become clear from experiments exchange substances untouched section where have not yet grown. GDH amin. AsAT greater anticipates Thus, wave actvities arise preceding KPA activity, however, localized site tube growth. The uptake into crude extract incorporation protein fraction were determined before different 14C-amino acids. Both pollination, there 14C-labelled acids fractions. proteins difficult metabolize acids, namely 14C-leucine 14C-threonine, 10 % but about same after. less 14C-proline 14C-protein hydrolysate taken up incorporated pollinated styles because portion easy decomposed or used wall synthesis. It evident differs plant, so one may speak isoenzymes. synthesized de-novo during Furthermore, activated under influence induces conformational molecule. By joining pollen- extract, deamin. undialyzable activator pollen. both NAD+ specific no activity.