The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpin.
作者： J F Kim , Z M Wei , S V Beer
DOI: 10.1128/JB.179.5.1690-1697.1997
关键词:
摘要: A 6.2-kb region of DNA corresponding to complementation groups II and III the Erwinia amylovora hrp gene cluster was analyzed. Transposon mutagenesis indicated that two are required for secretion harpin, an elicitor hypersensitive reaction. The sequence revealed 10 open reading frames in putative transcription units: hrpA, hrpB, hrcJ, hrpD, hrpE hrpA operon (group III) hrpF, hrpG, hrcC, hrpT, hrpV hrpC II). From promoter regions hrpC, hrpN operons, sequences similar those HrpL-dependent promoters Pseudomonas syringae pathovars were identified with a consensus 5'-GGAAC-N17-18-CACTNAA-3'. protein products seven genes, hrpE, hrpV, visualized T7 polymerase/promoter expression system. HrcC, HrcJ, HrpT contained potential signal peptides, HrcC appeared be envelope associated based on TnphoA translational fusion. Comparison deduced amino acid many proteins homologous function type pathway. is member YscC-containing subgroup PulD/pIV superfamily outer membrane proteins. HrcJ lipoprotein family includes YscJ Yersinia spp., MxiJ Shigella flexneri, NolT Rhizobim fredii. Additional similarities detected between HrpB YscI HrpE YscL. flagellar biogenesis FliF FliH, respectively. In addition, HrpA, HrpB, HrpD, HrpE, HrpF, showed various degrees similarity P. syringae. clusters respect organization individual confirms systems E. closely related each other distinct from Ralstonia (Pseudomonas) solanacearum Xanthomonas campestris. Possible implications extensive pathogenesis mechanisms discussed.
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