The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

摘要: The performance of immunosensing devices crucially depends on the methodology antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding antibodies are required. Herein, we introduce amine rich (NHx concentration 6 at.%) prepared by pulsed plasma polymerization cyclopropylamine (CPA) for functionalization of quartz crystal microbalance (QCM) surface the antibody to human serum albumin. In these the amine groups serve as anchor binding. The sensitivity QCM sensors in this way surpasses the one previously reported functionalized the thiolbased self-assembled monolayers factor 2. Our results thus show that CPA plasma polymers have a significant potential further development active for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.