Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions

摘要: Biosensors have been extensively developed and applied for biomedical environmental studies. Although there are many different types of biosensing techniques, immobilization the biorecognition biomolecules onto the sensor surface is always required. Cyclopropylamine pulsed plastha polymerization in radio frequency (RF) capacitive discharges was employed to deposit amine thin films on gold electrode quartz crystal microbalance (QCM) biosensors monoclonal antibody AL-01, specific to human serum albumin (HSA), was immobilized plasma polymer surface. Two different amine polymers were studied. They had a similar amount of primary groups, 13-1.5 at.%, as determined by chemical derivatization but their stability in water different. The 1st type, deposited at floating potential 120 Pa, exhibited 16% thickness loss after 24 h whereas the 2nd type RF 50 Pa completely stable (2% loss). Glutaraldehyde (GA) coupling of AL-01 for both the performance QCM immunosensors evaluated the immunoassay flow test A high-stability signal was obtained cases 2nd film provided low response HSA. It explained its highly cross-linked structure steric hindrance active sites. Three different antibody methods explored for 1st film. Stable baseline, selective and high recorded GA sulfo-SMCC methods. The results confirmed that presented methodologies the grafting highly efficient and very promising future use biosensing. (C) 2015 Elsevier B.V. All rights reserved.