Nucleic acid analysis techniques

摘要: The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. methods involve providing an array containing large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location each probe is known. Nucleic samples mRNA) from are hybridized to arrays pattern hybridization detected. Differences patterns indicates various genes those This also end-labeling acid. In one embodiment, involves acid, labeled then enzymatically ligating Thus, example, RNA, oligoribonucleotide can be ligated using RNA ligase. another end labeling accomplished by nucleoside triphosphates, attaching triphosphates terminal transferase.