Detection of polymorphisms in AFLP fragments using primer extension techniques
作者： Hanneke Witsenboer , Marius Tiemen Roelof Kupier
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摘要: The present invention relates to a method for determining the genotypes of polymorphic loci amplified in mixture restriction fragments, using an oligonucleotide sequence that is essentially complementary part target fragment, and located adjacent (upstream) polymorphism be detected. involves contacting fragments with under hybridization conditions, such when fragment present, hybrid formed between sequence. When resulting has at least one unpaired nucleotide being labeled or analog directly 3′ end sequence, extended analog, which can
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