xCITED2 Induces Neural Genes in Animal Cap Explants of Xenopus Embryos

摘要: Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression dominant negative BMP4 receptor (DNBR) produces neural in animal cap explants (AC) and also increases the level various genes involved neurogenesis. To investigate detail mechanism neurogenesis transcriptional level, we analyzed RNAs increased by DNBR using total RNA sequencing analysis identified several candidate genes. Among them, xCITED2 (Xenopus CBP/p300-interacting transcription activator) was induced 4.6 fold preferentially expressed tissues at tadpole stage. Ectopic anterior without mesoderm induction reduced BMP downstream genes, an eye specific marker posterior marker. Taken together, these results suggest may have a role differentiation