Molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by PCR and denaturing gradient gel electrophoresis.

摘要: Bacterial diversity in fluid milk products has been extensively studied order to improve quality. Here, we illustrate the utility of viable counts and PCR–denaturing gradient gel electrophoresis (DGGE) for monitoring microbial spoilage pasteurized during shelf life. Five samples stored at 4°C were examined 10 5 days before expiration on day. With bacterial DNA extracted directly from samples, PCR-DGGE analysis indicated that Pseudomonas became dominant four samples. Meanwhile, aerobic plate count these exceeded regulatory limit 20,000 CFU/ml expiration, rapid psychrotrophic markedly surpassed Streptococcus Buttiauxella spp. detected several Sequence DGGE fragments revealed high among P. putida migulae grew numbers refrigerated stora...