Evaluating the predictive value of doublecortin as a marker for adult neurogenesis in canaries (Serinus canaria)

摘要: Doublecortin (DCX) is an important microtubule-associated protein involved in the migration of young neurons into cortical layers brain during early human development. The continued expression DCX areas with protracted neuron recruitment has promoted this endogenous as a popular indirect tool to monitor adult neurogenesis variety species. However, little known about its possible involvement other cellular processes and thorough validation quantitative measure for generally lacking. Here we investigated relationship between brains canaries (Serinus canaria), species well-known neurogenesis. We examined age functional state DCX-labeled cells by using mitotic neuron-specific markers, retrograde tracings, immediate gene colocalizations. Although was high implicated neurogenesis, DCX-expressing were also abundant regions that do not recruit new neurons. Moreover, observed adult, active neurons, differentiated projection birth-dated up 1 year age. Season testosterone treatment affected two song control nuclei, HVC Area X, but did correlate patterns recruitment. Together, these results demonstrate exclusive migrating does predict equally throughout canary brain. Therefore, labeling needs careful each region separately analyzed when used quantify J. Comp. Neurol. 522:1299–1315, 2014. © 2013 Wiley Periodicals, Inc.