Hepatitis C virus (HCV) genotypes and chronic liver disease in Pakistan

摘要: UNLABELLED Hepatitis C virus (HCV) is classified into different types depending on nucleotide sequence variability. Detailed information the distribution of various HCV genotypes in some geographical areas available but little known about Pakistan. In this study, a 5' non-coding region (NCR)-based restriction fragment length polymorphism (RFLP) genotyping assay was used to investigate genotype large series HCV-infected patients Karachi, Serum samples from 74 hepatitis B surface antigen (HBsAg)-negative with clinical diagnosis chronic liver disease (60 patients) and hepatocellular carcinoma (HCC) (14 were assayed for anti-HCV antibody by second generation enzyme immunoassay 48 confirmed anti-HCV-positive (33 males, 15 females). Other causes (e.g. haemochromatosis, Wilson's immune-mediated injury) ruled out. Liver biopsy done 27/48 all HCC patients. Genotypes determined 45/48 study patients; 39/45 (87%) type 3; four (9%) 1; one 2; 5. Past blood transfusion main identifiable risk factor found 10 patients, 3. Seven 14 positive, (six 3). Most presented established cirrhosis complications portal hypertension failure. IN CONCLUSION (i) 3 most common isolate HCV-associated Pakistan; (ii) significant proportion HBsAg-negative cirrhotics are non-B, non-C aetiology; (iii) half have serological evidence infection.