Erythroid Progenitor Cells Expanded from Peripheral Blood without Mobilization or Preselection: Molecular Characteristics and Functional Competence

摘要: Background: Continued development of in-vitro procedures for expansion and differentiation erythroid progenitor cells (EPC) is essential not only in hematology stem cell research but also virology, light the strict erythrotropism clinically important human parvovirus B19. Methodology/Principal Findings: We cultured EPC directly from ordinary blood samples, without ex vivo mobilization or CD34+ vitro preselection. Profound increase absolute number clustering activity were observed during culture. The obtained expressed marker combination CD36, CD71 glycophorin, none lymphocyte, monocyte NK markers. functionality generated was examined by an infection assay with B19, tropic BFU-E CFU-E cells. Following (i) viral DNA replication mRNA production confirmed quantitative PCR, (ii) structural nonstructural proteins .50% As overall increased 100–200 fold, proportion competent (CD34+ to CD36+) rose ,0.5% .50%, culture procedure at efficiency .10 000-fold. Comparative culturing unselected PBMC vivo-preselected produced qualitatively quantitatively similar yields EPC. Conclusions/Significance: This approach yielding unmanipulated peripheral gratifyingly robust will facilitate study myeloid infectious agents such as B19 virus, well examination erythropoiesis its cellular molecular mechanisms.