Prevalence of Plasmodium falciparum Resistance Markers to Sulfadoxine-Pyrimethamine among Pregnant Women Receiving Intermittent Preventive Treatment for Malaria in Uganda

摘要: Background: The aim of this study was to assess prevalence mutations in the genes dihydrofolate reductase ( Pfdhfr ) and dihydropteroate synthase Pfdhps P. falciparum parasites among pregnant women using sulphadoxine-pyrimethamine (SP) as intermittent preventive treatment (IPTp). Methods: A molecular epidemiological parasite resistance markers conducted from August 2010 February 2012 Mukono district central Uganda. DNA extracted 413 positive samples. Real-time PCR followed by melting curve analysis (MCA) used characterize point Pfdhf r that are associated with SP resistance. Results: single nucleotide at codons 51I, 59R, 108N 437G 540E were high (>98%), reaching 100% fixation after one dose while 581G 3.3% baseline 12.5% SP. At baseline, Pfdhfr/Pfdhps quintuple 89%; sextuple (including 581G) low, 3.9%, 16.7% SP; but numbers infections follow-up visits small hence there insufficient statistical power test whether a true rise allele. overall frequency / throughout pregnancy excluded further analyses possible associations between certain haplotypes risk lower birth weight anaemia. However, infected had 1.3g/dl haemoglobin levels P =0.001) delivered babies 400gms compared non-parasitaemic women. Despite this, who become negative or two doses 44, 50.5% implying SP-IPTp still has some efficacy. Conclusions: population; infection poor outcomes.