Effect of variable concentration of serum on mouse embryo development.
作者： Hidekazu Saito , Trish Berger , Daniel R. Mishell , Richard P. Marrs
DOI: 10.1016/S0015-0282(16)47728-9
关键词:
摘要: This study was undertaken to determine the optimal concentration of serum necessary for maximal embryo development. Mouse embryos were cultured in vitro with 0% 30% concentrations 96hours. After 72 and 96hours culture, growth improved 5%, 10%, 20%, supplement when compared Ham's F-10 medium alone. Embryos then same 29hours, following which blastomere number, sister chromatid exchange (SCE), number micronuclei, chromosomal aberrations observed. There no difference any studied. All decreased SCE The rate or also smaller than that 5% serum. results these studies indicate should be employed culturing embryos, at least 10% is obtain conditions
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sci-hub.se 参考文章(19)
Alexander Lopata, Ian W.H. Johnston, Ian J. Hoult, Andrew I. Speirs, Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of a preovulatory egg. Fertility and Sterility. ,vol. 33, pp. 117- 120 ,(1980) , 10.1016/S0015-0282(16)44529-2
Richard P. Marrs, Joyce M. Vargyas, William E. Gibbons, Hidekazu Saito, Daniel R. Mishell, A modified technique of human in vitro fertilization and embryo transfer. American Journal of Obstetrics and Gynecology. ,vol. 147, pp. 318- 322 ,(1983) , 10.1016/0002-9378(83)91118-3
A.K. Tarkowski, An Air-Drying Method for Chromosome Preparations from Mouse Eggs Cytogenetic and Genome Research. ,vol. 5, pp. 394- 400 ,(1966) , 10.1159/000129914
P. Perry, H. J. Evans, Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature. ,vol. 258, pp. 121- 125 ,(1975) , 10.1038/258121A0
PAUL PERRY, SHELDON WOLFF, New Giemsa method for the differential staining of sister chromatids Nature. ,vol. 251, pp. 156- 158 ,(1974) , 10.1038/251156A0
A. V. CARRANO, L. H. THOMPSON, P. A. LINDL, J. L. MINKLER, Sister chromatid exchange as an indicator of mutagenesis Nature. ,vol. 271, pp. 551- 553 ,(1978) , 10.1038/271551A0
Motoi Ishidate, Shigeyoshi Odashima, Chromosome tests with 134 compounds on Chinese hamster cells in vitro — A screening for chemical carcinogens Mutation Research. ,vol. 48, pp. 337- 353 ,(1977) , 10.1016/0027-5107(77)90177-4
Charles J. Epstein, Sandra A. Smith, Amino acid uptake and protein synthesis in preimplantation mouse embryos Developmental Biology. ,vol. 33, pp. 171- 184 ,(1973) , 10.1016/0012-1606(73)90172-3
A. F. McFee, M. N. Sherrill, Mitotic response and sister chromatid exchanges in lymphocytes cultured in sera from different sources Experientia. ,vol. 37, pp. 27- 29 ,(1981) , 10.1007/BF01965549
R. L. BRINSTER, UPTAKE AND INCORPORATION OF AMINO ACIDS BY THE PREIMPLANTATION MOUSE EMBRYO Reproduction. ,vol. 27, pp. 329- 338 ,(1971) , 10.1530/JRF.0.0270329