Determination of photosynthetic and enzymatic biomarkers sensitivity used to evaluate toxic effects of copper and fludioxonil in alga Scenedesmus obliquus

摘要: Abstract Modulated PAM fluorometry and Plant Efficiency Analyser methods were used to investigate photosynthetic fluorescence parameters of alga Scenedesmus obliquus exposed inhibitory effect fungicides copper sulphate fludioxonil ( N -(4-nitrophenyl)- ′-propyl-uree). The change those studied when S. have been during 48 h different concentrations (1, 2 3 mg l −1 ). Under the same condition, enzymatic activities catalase, ascorbate peroxidase, glutathione reductase S -transferase investigated evaluate antioxidative response effects. sensitivity was dependent mode fungicide action, their concentration time exposure. For effects, most indicative biomarkers Q as non-photochemical quenching, Emax proton induced quenching ABS/RC antenna size per photosystem II reaction center. Copper oxidative stress indicated by increased activity catalase serving sensitive valuable biomarker. On other hand, on very negligible consequently not useful biomarkers. However, strong associated with cytosol enzymes, we found for peroxidase activities. By obtained results, may suggest activation enzymes be fludioxonil. Determination offer advantages in providing real criteria use them ecotoxicological diagnostic studies.