Construction and characterization of CV-1P cell lines which constitutively express the simian virus 40 agnoprotein: alteration of plaquing phenotype of viral agnogene mutants.

摘要: The simian virus 40 (SV40) agnoprotein is a 61-amino-acid polypeptide encoded in the leader region of some late viral mRNAs. Its function unclear, although previous investigations suggest that may transcriptional regulation and assembly. To define specific role(s) SV40 lytic cycle, CV-1P cell lines were constructed which agnogene was stably integrated constitutively expressed under control retroviral long terminal repeat. Two types isolated. One group, typified by line Ag18, produced low levels agnogene-specific mRNA agnoprotein. other type, represented single isolate named Ag8, high correspondingly By indirect immunofluorescence, located predominantly cytoplasmic perinuclear both lines; this its site localization wild-type (WT)-infected cells. Viruses agnoprotein-minus formed small plaques on normal cells, but nearly WT-sized Ag18 Conversely, Ag8 cells infected with mutants WT markedly smaller than these viruses when they grown Overall, our results trans-effector production. opposite effects plaquing observed correlated very different expression two lines. This suggests nature effect production vary depending intracellular concentration. Images