作者: Yunjun Zhao , Qian Zhang , Luxia Yuan , Rui Zhang , Laigeng Li
DOI: 10.4161/PSB.26956
关键词:
摘要: PtrMAN6 is a plant mannan endo-hydrolase involved in modulating cell expansion and wall thickening Populus developing xylem. N-glycosylation dimerization affect the enzymatic activity, which crucial for production of endogenous galactoglucomannan oligosaccharide signal molecule plants. There are 5 potential sites 6 cysteines sequence. Each or cysteine was site-direct mutagenized individually as well combination to analyze their effects on status enzyme activity. Our results demonstrated that all N-glycosylation, essential Meanwhile, we found only 3 carboxyl-terminal formation disulfide-linked dimer regulate The were conserved wall-bounded endo-hydrolases, this structure may...